unojУспехи наук о животныхErnst Journal of Animal Science3034-493ХФедеральный исследовательский центр животноводства – ВИЖ имени академика Л.К. Эрнста10.25687/3034-493X.2025.2.1.003unoj-11Research ArticleСтатьиЛиофилизация мужских гамет сельскохозяйственных животных и птиц: достижения, проблемы, перспективыLyophilization of male gametes of agricultural animals and birds: achievements, problems, prospectsКурочкинА. А.KurochkinA. A.

Санкт-Петербург

Saint Petersburg

ПритужаловаА. О.PrituzhalovaA. O.

Санкт-Петербург

Saint Petersburg

КузьминаТ. И.KuzminaT. I.

Санкт-Петербург

Saint Petersburg

СтанишевскаяО. И.StanishevskayaO. I.

Санкт-Петербург

Saint Petersburg

ВНИИГРЖ — филиал ФГБНУ ФИЦ ВИЖ им. Л.К. ЭрнстаРоссияAll-Russian Research Institute of Genetics and Farm Animal Breeding, Branch of L.K. Ernst Federal Research Center for Animal HusbandryRussian Federation202529072025013352Copyright © Курочкин А.А., Притужалова А.О., Кузьмина Т.И., Станишевская О.И., 20252025Курочкин А.А., Притужалова А.О., Кузьмина Т.И., Станишевская О.И.Kurochkin A.A., Prituzhalova A.O., Kuzmina T.I., Stanishevskaya O.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.ernstjournal.ru/jour/article/view/11

В настоящее время самым распространенным способом для длительного хранения репродуктивных клеток сельскохозяйственных животных и птиц является технология криоконсервации, которая предполагает хранение образцов в условиях сверхнизких температур. Однако значительные затраты на сохранность образцов и их транспортировку снижают показатели экономической эффективности метода. Лиофилизированная сперма может храниться при положительных температурах, и не требует для этого специального оборудования. На данный момент известно, что протоколы лиофилизации позволяют сохранять целостность хроматина сперматозоидов и факторы, детерминирующие активацию ооцитов у грызунов и млекопитающих, а метод интрацитоплазматической инъекции сперматозоида в яйцеклетку (ИКСИ) открывает возможность и перспективы использования «делиофилизированных» мужских гамет в репродуктивных технологиях. В статье представлен обзор литературы по проблеме сохранения спермы сельскохозяйственных животных в состоянии ангидробиоза. Описана история появления метода сублимационной сушки биологических образцов, проблемы предшествующих технологических этапов у разных видов сельскохозяйственных животных, резюмируются достижения и обозначаются тренды технологии лиофилизации мужских гамет животных

Currently, the most common method for long-term storage of reproductive cells for farm animals and birds is cryopreservation technology, which involves storing samples at ultra-low temperatures. However, significant costs for sample’s preservation and their transportation reduce economic efficiency of method. Lyophilized sperm can be stored at temperatures above zero and does not require special equipment for this. It is now known that lyophilization protocols make it possible to preserve the integrity of sperm chromatin and factors that determine oocyte activation in rodents and mammals, and method of intracytoplasmic sperm injection into the oocyte (ICSI) opens up possibility and prospects for using “lyophilized” male gametes in reproductive technologies. The article provides a literature review on problem of preserving farm animal’s sperm in a state of anhydrobiosis. Described history of the beginning freeze-drying method of biological samples, problems previous technological stages in different types of farm animals, summarized achievements and trends in technology of lyophilization of male animal gametes.

лиофилизациякриоконсервациякриобанкисперма Gallus gallusсперматозоидlyophilizationcryopreservationcryobanksGallus gallus spermspermatozoonReferencesBenedict, F.G. The determination of water in foods and physiological preparations / Benedict F.G., Manning C.R. // American journal of Physiology – Legacy Content. – 1905. – Vol. – 13. – № 3. – P. 309.Benedict, F.G. The determination of water in foods and physiological preparations / Benedict F.G., Manning C.R. // American journal of Physiology – Legacy Content. – 1905. – Vol. – 13. – № 3. – P. 309.Juan, S. R.-N. Liofilización de alimentos / Juan Sebastián Ramírez Navas. – Universidad del valle cali – Colombia. – 2007.Juan, S. R.-N. Liofilización de alimentos / Juan Sebastián Ramírez Navas. – Universidad del valle cali – Colombia. – 2007.Смит, О. Влияние низких температур на живые ткани и клетки. В кн.: Применение замораживания высушивания в биологии / Под ред. Р. Харриса. – Москва. – 1956. – 432 с.Смит, О. Влияние низких температур на живые ткани и клетки. В кн.: Применение замораживания высушивания в биологии / Под ред. Р. Харриса. – Москва. – 1956. – 432 с.Охапкина, В.Ю. Методы поддержания микробных культур. Часть 2. Лиофилизация / Теоретическая и прикладная экология. – 2009. – № 4. – С. 21.Охапкина, В.Ю. Методы поддержания микробных культур. Часть 2. Лиофилизация / Теоретическая и прикладная экология. – 2009. – № 4. – С. 21.Flosdorf, E.W. Studies with H. Pertussis: Maintenance of Cultures in Phase I / Flosdorf E.W., Kimball A. C. // Journal of Bacteriology. – 1939. – Vol. 39. – № 3. – P. 255.Flosdorf, E.W. Studies with H. Pertussis: Maintenance of Cultures in Phase I / Flosdorf E.W., Kimball A. C. // Journal of Bacteriology. – 1939. – Vol. 39. – № 3. – P. 255.Flosdorf, E.W. Procedure and apparatus for preservation in “lyophile” form of serum and other biological substances / Flosdorf E.W., Mudd S. // Journal of immunology. – 1935. – Vol. 29. – № 5. – P. 389.Flosdorf, E.W. Procedure and apparatus for preservation in “lyophile” form of serum and other biological substances / Flosdorf E.W., Mudd S. // Journal of immunology. – 1935. – Vol. 29. – № 5. – P. 389.Kupletskaya, M. B. Viability of lyophilized microorganisms after 50-year storage / Kupletskaya M. B., Netrusov A. I. // Microbiology. – 2011. – Vol. 80. – № 6. – P. 850.Kupletskaya, M. B. Viability of lyophilized microorganisms after 50-year storage / Kupletskaya M. B., Netrusov A. I. // Microbiology. – 2011. – Vol. 80. – № 6. – P. 850.Bond, C. Freeze-drying of yeast cultures / Bond C. // Methods in molecular biology. – 2007. – Vol. 368. – P. 99.Bond, C. Freeze-drying of yeast cultures / Bond C. // Methods in molecular biology. – 2007. – Vol. 368. – P. 99.Kawamura, S. Freeze-Drying of Yeasts / Kawamura S., Murakami Y., Miyamoto Y., Kimura K. // Cryopreservation and Freeze-Drying Protocols. – 1995. – P. 31.Kawamura, S. Freeze-Drying of Yeasts / Kawamura S., Murakami Y., Miyamoto Y., Kimura K. // Cryopreservation and Freeze-Drying Protocols. – 1995. – P. 31.Bunse, T. The preservation of fungal cultures by lyophilization / Bunse T., Steigleder G. K. // Mycoses. – 2009. – Vol. 34. – № 3-4. – P. 173.Bunse, T. The preservation of fungal cultures by lyophilization / Bunse T., Steigleder G. K. // Mycoses. – 2009. – Vol. 34. – № 3-4. – P. 173.Olaciregui, M. Freeze-dried spermatozoa: A future tool? / Olaciregui M., Gil L. // Reproduction in domestic Animals. – 2016. – Vol. 52. – P. 248.Olaciregui, M. Freeze-dried spermatozoa: A future tool? / Olaciregui M., Gil L. // Reproduction in domestic Animals. – 2016. – Vol. 52. – P. 248.Polge, C. Revival of Spermatozoa after Vitrification and Dehydration at Low Temperatures / Polge C., Smith A. U., Parkes A. S. // Nature. – 1949. – Vol. 164. – № 4172. – P. 666.Polge, C. Revival of Spermatozoa after Vitrification and Dehydration at Low Temperatures / Polge C., Smith A. U., Parkes A. S. // Nature. – 1949. – Vol. 164. – № 4172. – P. 666.Sherman, J. K. Freezing and freeze-drying of human spermatozoa / Sherman J. K., Jerome K. // Fertility and sterility. – 1954. – Vol. 5. – № 4. – P. 357.Sherman, J. K. Freezing and freeze-drying of human spermatozoa / Sherman J. K., Jerome K. // Fertility and sterility. – 1954. – Vol. 5. – № 4. – P. 357.Sherman, J. K. Freezing and freeze-drying of bull spermatozoa / Sherman J. K. // American journal of physiology-legacy content. – 1957. – Vol. 190. – № 2. – P. 281.Sherman, J. K. Freezing and freeze-drying of bull spermatozoa / Sherman J. K. // American journal of physiology-legacy content. – 1957. – Vol. 190. – № 2. – P. 281.Yushchenko, NP. Proof of the possibility of preserving mammalian spermatozoa in a dried state / Yushchenko NP. // Proc Lenin Acad Agr Sci. – 1957. – Vol. 22. – P. 37.Yushchenko, NP. Proof of the possibility of preserving mammalian spermatozoa in a dried state / Yushchenko NP. // Proc Lenin Acad Agr Sci. – 1957. – Vol. 22. – P. 37.Meryman, H. T. Survival of Spermatozoa Following Drying / Meryman H. T., Kafig E. // Nature. – 1959. – Vol. 184. – № 4684. – P. 470.Meryman, H. T. Survival of Spermatozoa Following Drying / Meryman H. T., Kafig E. // Nature. – 1959. – Vol. 184. – № 4684. – P. 470.Saacke, R. G. Freeze-drying of Bovine Spermatozoa / Saacke R. G., Almquist J. O. // Nature. – 1961. – Vol 192. – № 4806. – P. 995.Saacke, R. G. Freeze-drying of Bovine Spermatozoa / Saacke R. G., Almquist J. O. // Nature. – 1961. – Vol 192. – № 4806. – P. 995.Meryman, H. T. Special article freeze-drying of bovine spermatozoa / Meryman H. T., Kafig E. // Journal of reproduction and fertility. – 1963. – Vol 5. – № 1. – P. 87Meryman, H. T. Special article freeze-drying of bovine spermatozoa / Meryman H. T., Kafig E. // Journal of reproduction and fertility. – 1963. – Vol 5. – № 1. – P. 87Larson, E.V. Freeze-drying of spermatozoa / Larson E.V., Graham E.F. // Developments in biological standardization. – 1976. – Vol 36. – P. 343.Larson, E.V. Freeze-drying of spermatozoa / Larson E.V., Graham E.F. // Developments in biological standardization. – 1976. – Vol 36. – P. 343.Wakayama, T. Development of normal mice from oocytes injected with freeze-dried spermatozoa / Wakayama T., Yanagimachi R. // Nature biotechnology. – 1998. – Vol 16. – № 7. – P. 639.Wakayama, T. Development of normal mice from oocytes injected with freeze-dried spermatozoa / Wakayama T., Yanagimachi R. // Nature biotechnology. – 1998. – Vol 16. – № 7. – P. 639.Tselutin, K. Comparison of cryoprotectants and methods of cryopreservation of fowl spermatozoa / Tselutin K., Seigneurin F., Blesbois E. // Poultry science. – 1999. – Vol. 78. – № 4. – P. 586.Tselutin, K. Comparison of cryoprotectants and methods of cryopreservation of fowl spermatozoa / Tselutin K., Seigneurin F., Blesbois E. // Poultry science. – 1999. – Vol. 78. – № 4. – P. 586.Curry, MR. Cryopreservation of semen from domestic livestock / Curry MR. // Reviews of reproduction. – 2000. – Vol. 5. – № 1. – P. 46.Curry, MR. Cryopreservation of semen from domestic livestock / Curry MR. // Reviews of reproduction. – 2000. – Vol. 5. – № 1. – P. 46.Gliozzi, T. M. DNA fragmentation in chicken spermatozoa during cryopreservation / Gliozzi T. M., Zaniboni L., Cerolini S. // Theriogenology. – 2011. – Vol. 75. – № 9. – P. 1613.Gliozzi, T. M. DNA fragmentation in chicken spermatozoa during cryopreservation / Gliozzi T. M., Zaniboni L., Cerolini S. // Theriogenology. – 2011. – Vol. 75. – № 9. – P. 1613.Partyka, A. Evaluation of fresh and frozen-thawed fowl semen by flow cytometry / Partyka A., Niżański W., Łukaszewicz E. // Theriogenology. – 2010. – Vol. 74. – № 6. – P. 1019.Partyka, A. Evaluation of fresh and frozen-thawed fowl semen by flow cytometry / Partyka A., Niżański W., Łukaszewicz E. // Theriogenology. – 2010. – Vol. 74. – № 6. – P. 1019.Morris, J. G. Freezing injury: The special case of the sperm cell / Morris J. G., Acton E., Murray B. J., Fonseca F. // Cryobiology. – 2012. – Vol. 64. – № 2. – P. 71.Morris, J. G. Freezing injury: The special case of the sperm cell / Morris J. G., Acton E., Murray B. J., Fonseca F. // Cryobiology. – 2012. – Vol. 64. – № 2. – P. 71.Pegg, D.E. Principles of cryopreservation / Pegg D.E. // In: Wolkers W.F., Oldenhof H., Cryopreservation and Freeze-Drying Protocols, 3rd edn, Methods in Molecular Biology 1257, 2015. – P. 3–19.Pegg, D.E. Principles of cryopreservation / Pegg D.E. // In: Wolkers W.F., Oldenhof H., Cryopreservation and Freeze-Drying Protocols, 3rd edn, Methods in Molecular Biology 1257, 2015. – P. 3–19.Sieme, H. Sperm Membrane Behaviour during cooling and cryopreservation / Sieme H., Oldenhof H., Wolkers W. // Reproduction in domestic animals. – 2015. – Vol 50. – № 3. – P. 20.Sieme, H. Sperm Membrane Behaviour during cooling and cryopreservation / Sieme H., Oldenhof H., Wolkers W. // Reproduction in domestic animals. – 2015. – Vol 50. – № 3. – P. 20.Fraser, L. Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermatozoa following freezing–thawing / Fraser L., Strzeżek J. // Animal reproduction science. – 2007. – Vol. 99. – № 3-4. – P. 317.Fraser, L. Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermatozoa following freezing–thawing / Fraser L., Strzeżek J. // Animal reproduction science. – 2007. – Vol. 99. – № 3-4. – P. 317.Aitken, R. J. Reactive oxygen species as mediators of sperm capacitation and pathological damage / Aitken R. J. // Molecular reproduction and development. – 2017. – Vol. 84. – № 10. – P. 1039.Aitken, R. J. Reactive oxygen species as mediators of sperm capacitation and pathological damage / Aitken R. J. // Molecular reproduction and development. – 2017. – Vol. 84. – № 10. – P. 1039.Ford, C. Regulation of sperm function by reactive oxygen species / Ford C. // Human reproduction update. – 2004. – Vol. 10. – № 5. – P. 387.Ford, C. Regulation of sperm function by reactive oxygen species / Ford C. // Human reproduction update. – 2004. – Vol. 10. – № 5. – P. 387.Pons-Rejraji, H. Rôles des dérivés actifs de l’oxygène (DAO) sur les spermatozoïdes humains et infertilité masculine / Pons-Rejraji H., Sion B., Saez F., Brugnon F., Janny L., Grizard G. // Gynécologie obstétrique & Fertilité. – 2009. – Vol. 37. – №6. – P. 529.Pons-Rejraji, H. Rôles des dérivés actifs de l’oxygène (DAO) sur les spermatozoïdes humains et infertilité masculine / Pons-Rejraji H., Sion B., Saez F., Brugnon F., Janny L., Grizard G. // Gynécologie obstétrique & Fertilité. – 2009. – Vol. 37. – №6. – P. 529.Kowalczyk, A. Antioxidant effect of Elamipretide on bull’s sperm cells during freezing/thawing process / Kowalczyk A., Czerniawska Piątkowska E. // Andrology. – 2021. – Vol. 9. – № 4. – P. 1275.Kowalczyk, A. Antioxidant effect of Elamipretide on bull’s sperm cells during freezing/thawing process / Kowalczyk A., Czerniawska Piątkowska E. // Andrology. – 2021. – Vol. 9. – № 4. – P. 1275.Kläver, R. Routine cryopreservation of spermatozoa is safe — Evidence from the DNA methylation pattern of nine spermatozoa genes / Kläver R., Bleiziffer A., Redmann K., Mallidis C., Kliesch S., Gromoll J. // Journal of assisted reproduction and genetics. – 2012. – Vol. 29. – № 9. – P. 943.Kläver, R. Routine cryopreservation of spermatozoa is safe — Evidence from the DNA methylation pattern of nine spermatozoa genes / Kläver R., Bleiziffer A., Redmann K., Mallidis C., Kliesch S., Gromoll J. // Journal of assisted reproduction and genetics. – 2012. – Vol. 29. – № 9. – P. 943.Aitken, R.J. Biological and clinical significance of DNA damage in the male germ line / Aitken R.J., De Luliis G.N., McLachlan R.I. // International journal of andrology. – 2008. – Vol 32. – № 1. – P. 46.Aitken, R.J. Biological and clinical significance of DNA damage in the male germ line / Aitken R.J., De Luliis G.N., McLachlan R.I. // International journal of andrology. – 2008. – Vol 32. – № 1. – P. 46.Agarwal, A. Role of reactive oxygen species in the pathophysiology of human reproduction / Agarwal A., Saleh R.A., Bedaiwy M.A. // Fertility and sterility. – 2003. – Vol 79. – № 4. – P. 829.Agarwal, A. Role of reactive oxygen species in the pathophysiology of human reproduction / Agarwal A., Saleh R.A., Bedaiwy M.A. // Fertility and sterility. – 2003. – Vol 79. – № 4. – P. 829.Thomson, L.K. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis / Thomson L.K., Fleming S.D., Aitken R.J., De luliis G.N., Zieschang J.A., Clark A.M. // Human reproduction. – 2009. – Vol. 24. – № 9. – P. 2061.Thomson, L.K. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis / Thomson L.K., Fleming S.D., Aitken R.J., De luliis G.N., Zieschang J.A., Clark A.M. // Human reproduction. – 2009. – Vol. 24. – № 9. – P. 2061.Плешанов Н. В. Криоустойчивость спермы петухов в зависимости от содержания в ней липидов / Плешанов Н. В., Станишевская О. И., Силюкова Ю.Л. // Генетика и разведение животных. – 2017. – № 3. – С. 34.Плешанов Н. В. Криоустойчивость спермы петухов в зависимости от содержания в ней липидов / Плешанов Н. В., Станишевская О. И., Силюкова Ю.Л. // Генетика и разведение животных. – 2017. – № 3. – С. 34.Surai, P. Polyunsaturated fatty acids, lipid peroxidation and antioxidant protection in avian semen / Surai P., Fujihara N., Speake B., Brillard J., Wishart G., Sparks N. //Asian-Australasian journal of animal sciences. 2001. – Vol. 17. – № 7. – P. 1024.Surai, P. Polyunsaturated fatty acids, lipid peroxidation and antioxidant protection in avian semen / Surai P., Fujihara N., Speake B., Brillard J., Wishart G., Sparks N. //Asian-Australasian journal of animal sciences. 2001. – Vol. 17. – № 7. – P. 1024.Partyka, A. Supplementation of Avian Semen Extenders with Antioxidants to Improve Semen Quality—Is It an Effective Strategy? / Partyka A., Niżański W. // Antioxidants. – 2021. – Vol. 10. – № 12.Partyka, A. Supplementation of Avian Semen Extenders with Antioxidants to Improve Semen Quality—Is It an Effective Strategy? / Partyka A., Niżański W. // Antioxidants. – 2021. – Vol. 10. – № 12.Gil, L. Current status of freeze-drying technology to preserve domestic animals sperm / Gil L., Olaciregui M., Luño V., Malo C., González N., Martínez F. // Reproduction in domestic animals. – 2014. – Vol 49. – № 4. – P. 72.Gil, L. Current status of freeze-drying technology to preserve domestic animals sperm / Gil L., Olaciregui M., Luño V., Malo C., González N., Martínez F. // Reproduction in domestic animals. – 2014. – Vol 49. – № 4. – P. 72.Olaciregui, M. Freeze dried stallion spermatozoa: Evaluation of two chelating agents and comparative analysis of three sperm DNA damage assays / Olaciregui M., Luño V., Marti J. I., Aramayona J., Gil L. //Andrologia. – 2015. – Vol. 48. – № 9. – P. 988.Olaciregui, M. Freeze dried stallion spermatozoa: Evaluation of two chelating agents and comparative analysis of three sperm DNA damage assays / Olaciregui M., Luño V., Marti J. I., Aramayona J., Gil L. //Andrologia. – 2015. – Vol. 48. – № 9. – P. 988.Nakai, M. Effects of chelating agents during freeze-drying of boar spermatozoa on DNA fragmentation and on developmental ability in vitro and in vivo after intracytoplasmic sperm head injection / Nakai M., Kashiwazaki N., Takizawa A., Maedomari N., Ozawa M., Noguchi J., Kaneko H., Shino M., Kikuchi K. // Zygote. – 2007. – Vol 15. – № 1. – P. 15.Nakai, M. Effects of chelating agents during freeze-drying of boar spermatozoa on DNA fragmentation and on developmental ability in vitro and in vivo after intracytoplasmic sperm head injection / Nakai M., Kashiwazaki N., Takizawa A., Maedomari N., Ozawa M., Noguchi J., Kaneko H., Shino M., Kikuchi K. // Zygote. – 2007. – Vol 15. – № 1. – P. 15.Kusakabe, H. Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa / Kusakabe H., Szczygiel M. A., Whittingham D. G., Yanagimachi R. // Proceedings of the national academy of sciences. – 2001. – Vol. 98. – № 24. – P. 13501.Kusakabe, H. Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa / Kusakabe H., Szczygiel M. A., Whittingham D. G., Yanagimachi R. // Proceedings of the national academy of sciences. – 2001. – Vol. 98. – № 24. – P. 13501.Hara, H. Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa / Hara H., Tagiri M., Hwang I. S., Takahashi M., Hirabayasi M. // Cryobiology. – 2014. – Vol. 68. – № 3. – P. 354.Hara, H. Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa / Hara H., Tagiri M., Hwang I. S., Takahashi M., Hirabayasi M. // Cryobiology. – 2014. – Vol. 68. – № 3. – P. 354.Crowe, J. H. Anhydrobiosis / Crowe J. H., Hoekstra F. A., Crowe L. M. // Annual review of physiology. – 1992. – Vol. 54. – № 1. – P. 579.Crowe, J. H. Anhydrobiosis / Crowe J. H., Hoekstra F. A., Crowe L. M. // Annual review of physiology. – 1992. – Vol. 54. – № 1. – P. 579.Wolfe, J. Freezing, drying, and/or vitrification of membrane– solute–water systems / Wolfe J., Bryant G. // Cryobiology. – 1999. – Vol 39. – № 2. – P. 103.Wolfe, J. Freezing, drying, and/or vitrification of membrane– solute–water systems / Wolfe J., Bryant G. // Cryobiology. – 1999. – Vol 39. – № 2. – P. 103.Wolkers, W. F., Oldenhof, H. Cryopreservation and Freeze-Drying Protocols. - Methods in Molecular Biology, Springer, New York, 2021 – 742 p.Wolkers, W. F., Oldenhof, H. Cryopreservation and Freeze-Drying Protocols. - Methods in Molecular Biology, Springer, New York, 2021 – 742 p.Martins, C. F. Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm / Martins C. F., Báo S. N., Dode M. N., Correa G. A., Rumpf R. // Theriogenology. 2007. – Vol. 67. – № 8. – P. 1307.Martins, C. F. Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm / Martins C. F., Báo S. N., Dode M. N., Correa G. A., Rumpf R. // Theriogenology. 2007. – Vol. 67. – № 8. – P. 1307.Men, N. T. Effect of trehalose on DNA integrity of freeze-dried boar sperm, fertilization, and embryo development after intracytoplasmic sperm injection / Men N. T., Kikuchi K., Nakai M., Fukuda A., Tanihara F., Noguchi J., Kaneko H., Linh V. N., Nguyen B.X., Nagai T., Tajima A. // Theriogenology. – 2013. – Vol 80. – № 9. – P. 1033.Men, N. T. Effect of trehalose on DNA integrity of freeze-dried boar sperm, fertilization, and embryo development after intracytoplasmic sperm injection / Men N. T., Kikuchi K., Nakai M., Fukuda A., Tanihara F., Noguchi J., Kaneko H., Linh V. N., Nguyen B.X., Nagai T., Tajima A. // Theriogenology. – 2013. – Vol 80. – № 9. – P. 1033.Stanishevskaya, O. A successful protocol for achieving anhydrobiosis of Gallus Gallus Domesticus spermatozoa while maintaining their fertility IN VIVO / Stanishevskaya O., Silyukova Y., Pleshanov N., Kurochkin A., Fedorova E., Radaev A. // Cryobiology. – 2022. – Vol. 104. – P. 102.Stanishevskaya, O. A successful protocol for achieving anhydrobiosis of Gallus Gallus Domesticus spermatozoa while maintaining their fertility IN VIVO / Stanishevskaya O., Silyukova Y., Pleshanov N., Kurochkin A., Fedorova E., Radaev A. // Cryobiology. – 2022. – Vol. 104. – P. 102.Usui, T. Cytotoxicity and oxidative stress induced by the glyceraldehyde-related maillard reaction products for HL-60 cells / Usui T., Shizuuchi S., Watanabe H., Hayase F. // Bioscience, biotechnology, and biochemistry. – 2004. – Vol 68. – № 2. – P. 333.Usui, T. Cytotoxicity and oxidative stress induced by the glyceraldehyde-related maillard reaction products for HL-60 cells / Usui T., Shizuuchi S., Watanabe H., Hayase F. // Bioscience, biotechnology, and biochemistry. – 2004. – Vol 68. – № 2. – P. 333.Edeas, M. Maillard Reaction, mitochondria and oxidative stress: potential role of antioxidants / Edeas M., Attaf D., Mailfert A.-S., Nasu M., Joubet R. // Pathologie Biologie. – 2010. – Vol. 58. – № 3. – P. 220.Edeas, M. Maillard Reaction, mitochondria and oxidative stress: potential role of antioxidants / Edeas M., Attaf D., Mailfert A.-S., Nasu M., Joubet R. // Pathologie Biologie. – 2010. – Vol. 58. – № 3. – P. 220.Drobnis, E. Z. Cold shock damage is due to lipid phase transitions in cell membranes: A demonstration using sperm as a model / Drobnis E. Z., Crowe L. M., Berger T., Anchordoguy T. J., Overstreet J. W., Crowe J. H. // Journal of experimental zoology. – 1993. – Vol 265. – № 4. – P. 432.Drobnis, E. Z. Cold shock damage is due to lipid phase transitions in cell membranes: A demonstration using sperm as a model / Drobnis E. Z., Crowe L. M., Berger T., Anchordoguy T. J., Overstreet J. W., Crowe J. H. // Journal of experimental zoology. – 1993. – Vol 265. – № 4. – P. 432.Van Winden, E. C. Freeze-Drying of Liposomes: Theory and Practice / Van Winden E. C. // Methods in enzymology. – 2003. – Vol. 367. – P. 99-110.Van Winden, E. C. Freeze-Drying of Liposomes: Theory and Practice / Van Winden E. C. // Methods in enzymology. – 2003. – Vol. 367. – P. 99-110.Crowe, J. H. Preservation of dry liposomes does not require retention of residual water / Crowe J. H., Spargo B. J., Crowe L. M. // Proceedings of the National Academy of Sciences. – 1987. – Vol. 84. – № 6. – P. 1537.Crowe, J. H. Preservation of dry liposomes does not require retention of residual water / Crowe J. H., Spargo B. J., Crowe L. M. // Proceedings of the National Academy of Sciences. – 1987. – Vol. 84. – № 6. – P. 1537.França, M. B. Oxidative stress and its effects during dehydration / França M. B., Panek A. D., Eleutherio E. C. A. // Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. – 2007. – Vol. 146. – № 4. – P. 621.França, M. B. Oxidative stress and its effects during dehydration / França M. B., Panek A. D., Eleutherio E. C. A. // Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. – 2007. – Vol. 146. – № 4. – P. 621.Карбышев, М.С. Биохимия оксидативного стресса: учеб. метод. пособие / Карбышев М.С., Абдулаев Ш.П. – Москва: ФГБОУ ВО РНИМУ, 2018. – 60 с.Карбышев, М.С. Биохимия оксидативного стресса: учеб. метод. пособие / Карбышев М.С., Абдулаев Ш.П. – Москва: ФГБОУ ВО РНИМУ, 2018. – 60 с.Kusakabe, H. Chromosomal integrity and DNA damage in freeze-dried spermatozoa / Kusakabe H. // Reproductive medicine and biology. – 2011. – Vol. 10. – № 4. – P. 199.Kusakabe, H. Chromosomal integrity and DNA damage in freeze-dried spermatozoa / Kusakabe H. // Reproductive medicine and biology. – 2011. – Vol. 10. – № 4. – P. 199.Kusakabe, H. Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa / Kusakabe H., Szczygiel M. A., Whittingham D. G., Yanagimachi R. // Proceedings of the National Academy of Sciences. – 2001. – Vol. 98. – № 24. – P. 13501.Kusakabe, H. Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa / Kusakabe H., Szczygiel M. A., Whittingham D. G., Yanagimachi R. // Proceedings of the National Academy of Sciences. – 2001. – Vol. 98. – № 24. – P. 13501.Bakst, M. R. The inner perivitelline layer sperm hole assay: Use of filter paper rings for the isolation of the perivitelline layer overlying the germinal disc and new observations on its morphology / Bakst M. R., Eastridge J., Malecki I. A. // The journal of applied poultry research. 2014. – Vol. 23. – № 1. – P. 121.Bakst, M. R. The inner perivitelline layer sperm hole assay: Use of filter paper rings for the isolation of the perivitelline layer overlying the germinal disc and new observations on its morphology / Bakst M. R., Eastridge J., Malecki I. A. // The journal of applied poultry research. 2014. – Vol. 23. – № 1. – P. 121.Restrepo, G. Freezing, vitrification and freeze-drying of equine spermatozoa: impact on mitochondrial membrane potential, lipid peroxidation and DNA integrity / Restrepo G., Varela E., Duque J. E., Gómez J. E., Rojas M. // Journal of Equine Veterinary Science. – 2018. – Vol. 72. – P. 8.Restrepo, G. Freezing, vitrification and freeze-drying of equine spermatozoa: impact on mitochondrial membrane potential, lipid peroxidation and DNA integrity / Restrepo G., Varela E., Duque J. E., Gómez J. E., Rojas M. // Journal of Equine Veterinary Science. – 2018. – Vol. 72. – P. 8.Oldenhof, H. Freezing-induced uptake of disaccharides for preservation of chromatin in freeze-dried stallion sperm during accelerated aging / Oldenhof H., Zhang, M., Narten K., Bigalk J., Sydykov B., Wolkers W. F., Sieme H. // Biology of reproduction. – 2017. – Vol. 97. – № 6. – P. 892.Oldenhof, H. Freezing-induced uptake of disaccharides for preservation of chromatin in freeze-dried stallion sperm during accelerated aging / Oldenhof H., Zhang, M., Narten K., Bigalk J., Sydykov B., Wolkers W. F., Sieme H. // Biology of reproduction. – 2017. – Vol. 97. – № 6. – P. 892.Olaciregui, M. Chelating agents in combination with rosmarinic acid for boar sperm freezedrying / Olaciregui M., Luño V., González N., Domingo P., Blas I., Gil L. // Reproductive Biology. – 2017. – Vol. 17. – № 3. – P. 193.Olaciregui, M. Chelating agents in combination with rosmarinic acid for boar sperm freezedrying / Olaciregui M., Luño V., González N., Domingo P., Blas I., Gil L. // Reproductive Biology. – 2017. – Vol. 17. – № 3. – P. 193.Palazzese, L. DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development / Palazzese L., Gosálvez J., Anzalone D. A., Loi P., Saragusty J. // Journal of reproduction and development. – 2018. – Vol. 64. – № 5. – P. 393.Palazzese, L. DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development / Palazzese L., Gosálvez J., Anzalone D. A., Loi P., Saragusty J. // Journal of reproduction and development. – 2018. – Vol. 64. – № 5. – P. 393.Arav, A. High post-thaw survival of ram sperm after partial freeze-drying / Arav A., Idda A., Nieddu S. M., Natan Y., Ledda S. // Journal of Assisted Reproduction and Genetics. – 2018. – Vol. 35. – № 7. – P. 1149.Arav, A. High post-thaw survival of ram sperm after partial freeze-drying / Arav A., Idda A., Nieddu S. M., Natan Y., Ledda S. // Journal of Assisted Reproduction and Genetics. – 2018. – Vol. 35. – № 7. – P. 1149.Anzalone, D. A. Freeze-dried spermatozoa: An alternative biobanking option for endangered species / Anzalone D. A., Palazzese L., Iuso D., Martino G., Loi P. // Animal reproduction science. – 2018. – Vol. 190. – P. 85.Anzalone, D. A. Freeze-dried spermatozoa: An alternative biobanking option for endangered species / Anzalone D. A., Palazzese L., Iuso D., Martino G., Loi P. // Animal reproduction science. – 2018. – Vol. 190. – P. 85.Olaciregui, M. In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa / Olaciregui M., Luño V., Domingo P., González N., Gil L. // Scientific Reports. – 2017. – Vol. 7. – № 1.Olaciregui, M. In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa / Olaciregui M., Luño V., Domingo P., González N., Gil L. // Scientific Reports. – 2017. – Vol. 7. – № 1.Wakayama, S. Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months / Wakayama S., Tada M., Osada I., Nagamatsu A., Kamimura S., Nagatomo H., Mizutani E., Ishino F., Yano S., Wakayama, T. // Proceedings of the National Academy of Sciences. – 2017. – Vol. 114. – № 23. – P. 5988.Wakayama, S. Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months / Wakayama S., Tada M., Osada I., Nagamatsu A., Kamimura S., Nagatomo H., Mizutani E., Ishino F., Yano S., Wakayama, T. // Proceedings of the National Academy of Sciences. – 2017. – Vol. 114. – № 23. – P. 5988.Ito, D. Mailing viable mouse freeze-dried spermatozoa on postcards / Ito D., Wakayama S., Emura R., Ooga M., Wakayama T. // iScience. – 2021. – Vol. 24. – № 8.Ito, D. Mailing viable mouse freeze-dried spermatozoa on postcards / Ito D., Wakayama S., Emura R., Ooga M., Wakayama T. // iScience. – 2021. – Vol. 24. – № 8.Kamada, Y. Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum / Kamada Y., Wakayama S., Shibasaki I., Ito D., Kamimura S., Ooga M., Wakayama T. // Scientific Reports. – 2018. Vol. 8. – № 1.Kamada, Y. Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum / Kamada Y., Wakayama S., Shibasaki I., Ito D., Kamimura S., Ooga M., Wakayama T. // Scientific Reports. – 2018. Vol. 8. – № 1.Kaneko, T. Long-term preservation of freeze-dried mouse spermatozoa / Kaneko T., Serikawa T. // Cryobiology. – 2012. – Vol. 64. – № 3. – P. 211.Kaneko, T. Long-term preservation of freeze-dried mouse spermatozoa / Kaneko T., Serikawa T. // Cryobiology. – 2012. – Vol. 64. – № 3. – P. 211.Kaneko, T. Successful Long-Term Preservation of Rat Sperm by Freeze-Drying / Kaneko, T., Serikawa, T. // PLoS ONE. – 2012. – Vol. 7. – № 4.Kaneko, T. Successful Long-Term Preservation of Rat Sperm by Freeze-Drying / Kaneko, T., Serikawa, T. // PLoS ONE. – 2012. – Vol. 7. – № 4.Liu, J.-L. Freeze-Dried Sperm Fertilization Leads to Full-Term Development in Rabbits / Liu J.-L., Kusakabe H., Chang C.-C., Suzuki H., Schmidt D. W., Julian M., Pfeffer R., Bormann C.L, Tian X.C., Yanagimachi R., Yang X. // Biology of reproduction. – 2004. – Vol. 70. – № 6. – P. 1776.Liu, J.-L. Freeze-Dried Sperm Fertilization Leads to Full-Term Development in Rabbits / Liu J.-L., Kusakabe H., Chang C.-C., Suzuki H., Schmidt D. W., Julian M., Pfeffer R., Bormann C.L, Tian X.C., Yanagimachi R., Yang X. // Biology of reproduction. – 2004. – Vol. 70. – № 6. – P. 1776.Kwon, I.-K. Activation, pronuclear formation, and development in vitro of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa / Kwon I.-K., Park K.-E., Niwa K. // Biology of reproduction. – 2004. – Vol. 71. – №5. – P. 1430.Kwon, I.-K. Activation, pronuclear formation, and development in vitro of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa / Kwon I.-K., Park K.-E., Niwa K. // Biology of reproduction. – 2004. – Vol. 71. – №5. – P. 1430.Li, X.-X. Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa / Li X.-X., Diao Y.-F., Wei H.- J., Wang S.-Y., Cao X.-Y., Zhang Y.-F., Tong C., Li D.-L., Kim M.K., Xu, B. // Scientific Reports. – 2017. – Vol 7. – № 1.Li, X.-X. Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa / Li X.-X., Diao Y.-F., Wei H.- J., Wang S.-Y., Cao X.-Y., Zhang Y.-F., Tong C., Li D.-L., Kim M.K., Xu, B. // Scientific Reports. – 2017. – Vol 7. – № 1.Choi, Y. H. Production of live foals via intracytoplasmic injection of lyophilized sperm and sperm extract in the horse / Choi Y. H., Varner D. D., Love C. C., Hartman D. L., Hinrichs K. // Reproduction. – 2011. – Vol. 142. – № 4. – P. 529.Choi, Y. H. Production of live foals via intracytoplasmic injection of lyophilized sperm and sperm extract in the horse / Choi Y. H., Varner D. D., Love C. C., Hartman D. L., Hinrichs K. // Reproduction. – 2011. – Vol. 142. – № 4. – P. 529.Keskintepe, L. Bovine blastocyst development from oocytes injected with freeze-dried spermatozoa / Keskintepe L., Pacholczyk G., Machnicka A., Norris K., Curuk M. A., Khan I., Brackett B. G. // Biology of Reproduction. – 2002. – Vol. 67. – № 2. – P. 409.Keskintepe, L. Bovine blastocyst development from oocytes injected with freeze-dried spermatozoa / Keskintepe L., Pacholczyk G., Machnicka A., Norris K., Curuk M. A., Khan I., Brackett B. G. // Biology of Reproduction. – 2002. – Vol. 67. – № 2. – P. 409.Palazzese, L. Whole genome integrity and enhanced developmental potential in ram freeze-dried spermatozoa at mild sub-zero temperature / Palazzese L., Anzalone D. A., Turri F., Faieta M., Donnadio A., Pizzi F., Pittia P., Matsukawa K., Loi P. // Scientific Reports. – 2020. – Vol 10. – № 1.Palazzese, L. Whole genome integrity and enhanced developmental potential in ram freeze-dried spermatozoa at mild sub-zero temperature / Palazzese L., Anzalone D. A., Turri F., Faieta M., Donnadio A., Pizzi F., Pittia P., Matsukawa K., Loi P. // Scientific Reports. – 2020. – Vol 10. – № 1.Thiangthientham, P. Effects of freeze-drying on the quality and fertilising ability of goat sperm recovered from different parts of the epididymis / Thiangthientham P., Kallayanathum W., Anakkul N., Suwimonteerabutr J., Santiviparat S., Techakumphu M., Loi P., Tharasanit T. // Theriogenology. – 2023. – Vol. 195. – № 1. – P. 31.Thiangthientham, P. Effects of freeze-drying on the quality and fertilising ability of goat sperm recovered from different parts of the epididymis / Thiangthientham P., Kallayanathum W., Anakkul N., Suwimonteerabutr J., Santiviparat S., Techakumphu M., Loi P., Tharasanit T. // Theriogenology. – 2023. – Vol. 195. – № 1. – P. 31.González-Marín, C. Types, causes, detection and repair of DNA fragmentation in animal and human sperm cells / González-Marín C., Gosálvez J., Roy R. // International journal of molecular sciences. – 2012. – Vol. 13. – № 12. – P. 14026.González-Marín, C. Types, causes, detection and repair of DNA fragmentation in animal and human sperm cells / González-Marín C., Gosálvez J., Roy R. // International journal of molecular sciences. – 2012. – Vol. 13. – № 12. – P. 14026.Patrick, J., Comizzoli P., Elliott G. Dry preservation of spermatozoa: considerations for different Species / Patrick, J., Comizzoli, P., Elliott, G. // Biopreservation and biobanking. – 2017. – Vol. 15. – № 2. – P. 158.Patrick, J., Comizzoli P., Elliott G. Dry preservation of spermatozoa: considerations for different Species / Patrick, J., Comizzoli, P., Elliott, G. // Biopreservation and biobanking. – 2017. – Vol. 15. – № 2. – P. 158.Hirabayashi, M. Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads / Hirabayashi M., Kato M., Ito J., Hochi S. // Zygote. – 2005. – Vol. 13. – № 1. – P. 79.Hirabayashi, M. Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads / Hirabayashi M., Kato M., Ito J., Hochi S. // Zygote. – 2005. – Vol. 13. – № 1. – P. 79.Kaneko, T. Offspring derived from oocytes injected with rat sperm, frozen or freeze-dried without cryoprotection / Kaneko T., Kimura S., Nakagata N. // Theriogenology. – 2007. – Vol. 68. – № 7. – P. 1017.Kaneko, T. Offspring derived from oocytes injected with rat sperm, frozen or freeze-dried without cryoprotection / Kaneko T., Kimura S., Nakagata N. // Theriogenology. – 2007. – Vol. 68. – № 7. – P. 1017.Rajska, I. Freeze Drying as a Method of Long-Term Conservation of Mammalian Semen – A Review / Rajska I. // Annals of Animal Science. – 2021. – Vol. 21. – № 4.Rajska, I. Freeze Drying as a Method of Long-Term Conservation of Mammalian Semen – A Review / Rajska I. // Annals of Animal Science. – 2021. – Vol. 21. – № 4.

The authors declare that there are no conflicts of interest present.